Effect of sludge redistribution strategy on stability of partial nitrification-anammox process: Further exploration of the potential value of sludge.

The stability of the two-stage partial nitrification-anammox (PN/A) system was compromised by the inappropriate conversion of insoluble organic matter. In response, a sludge redistribution strategy was implemented. Through the redistribution of PN sludge and anammox sludge in the two-stage PN/A system, a transition was made to the Anammox-single stage PN/A (A-PN/A) system. This specific functional reorganization, facilitated by the rapid reorganization of microbial communities, has the potential to significantly decrease the current risk of suppression. The results of the study showed that implementing the sludge redistribution strategy led to a substantial enhancement in the total nitrogen removal rate (TNRR) by 87.51%, accompanied by a significant improvement of 34.78% in the chemical oxygen demand removal rate (CRR). Additionally, this approach resulted in a remarkable two-thirds reduction in the aeration requirements. High-throughput sequencing revealed that the strategy enriched anammox and ammonia-oxidizing bacteria while limiting denitrifying bacteria, as confirmed by quantitative polymerase chain reaction analysis. Furthermore, the principal component analysis revealed that the location and duration of aeration had direct and indirect effects on functional gene expression and the evolution of microbial communities. This study emphasizes the potential benefits of restructuring microbial communities through a sludge redistribution strategy, especially in integrated systems that encounter challenges with suppression.

Natural lighting enhancing the algae proliferation and nitrogen removal in membrane-aerated bacterial-algal biofilm reactor.

Membrane-aerated bacterial-algal biofilm reactor (MABAR) is an emerging and novel technology in recent years, which has been attracting increasing attention due to its cost-effectiveness and superior removal performance of pollutants by versatile removal pathways in symbiotic bacterial-algal biofilm. However, the wider application of MABAR is hindered by the dilemma of insufficient algae biomass. In this study, an MABAR under natural sunlight was developed and operated for 160 d to access the feasibility of enhancing algae proliferation by natural lighting. Results showed that the MABAR with natural sunlight (nMABAR) demonstrated better performance of pollutants removal. High removal efficiencies of organic matter and NH4-N in nMABAR were 90 % and 92 %, respectively. In particular, the removal efficiency of TN in nMABAR, under less aeration, was up to 80 %, which was 15 % higher than the control reactor. The Chlorophyll-a content indicated that natural sunlight facilitated to algae growth in MABAR, and algae assimilation might be the dominant contributor to NH4-N removal. Moreover, there were microbial shifts in bacterial-algal biofilm in a response to the natural lighting, the nMABAR uniquely possessed a bacterial phylotype termed Thiocapsa, which could play an important role in bacterial nitrification. Algal phylotype Chlorophyceae significantly contributed to pollutants removal and synergistic relationship with bacteria. In addition, the superb performance of nMABAR under less aeration condition suggested that abundant algae were capable of supplying enough O2 for the system. These results provided insight into the natural lighting on algae-bacteria synergistic growth and cost-effective operation strategy for MABAR.